############# Model summary Number of compounds: 2053 Number of reactions: 1943 Number of genes: 1701 Ratio rxn with genes/rxns: 83% ############# Analyzing targets #Topological analysis Number of targets: 84 Reading draft network from /shared/chondrus/networks/chondrus_final.sbml ... done. Reading seeds from growth_medium/seeds_artefacts.sbml ... done. Reading targets from targets_compounds/targets.sbml ... done. Checking draftnet for unproducible targets ... done. 55 producible targets: "M_CPD__45__14553_c" "M_MEK_c" "M_CPD1F__45__130_c" "M_BUTANOL_c" "M_ACETONE_c" "M_CPD__45__7830_c" "M_ISOBUTYRATE_c" "M_ARACHIDONIC_ACID_c" "M_CPD__45__7031_c" "M_D__45__Galactosyl__45__12__45__diacyl__45__glycerols_c" "M_CPD__45__9245_c" "M_TYR_c" "M_HEXANAL_c" "M_STEARIC_ACID_c" "M_CPD__45__7875_c" "M_LEU_c" "M_TETRACOSANOATE_c" "M_CPD1F__45__129_c" "M_THR_c" "M_MET_c" "M_CPD__45__18778_c" "M_PALMITATE_c" "M_LYS_c" "M_BENZALDEHYDE_c" "M_ILE_c" "M_HIS_c" "M_ACETALD_c" "M_GLY_c" "M_L__45__ASPARTATE_c" "M_ARACHIDIC_ACID_c" "M_L__45__ALPHA__45__ALANINE_c" "M_CPD__45__14646_c" "M_ETOH_c" "M_CPD__45__681_c" "M_CYSTINE_c" "M_GLT_c" "M_CPD__45__7836_c" "M_L__45__ORNITHINE_c" "M_METHYLBUT__45__CPD_c" "M_ARG_c" "M_DODECANOATE_c" "M_CPD__45__7845_c" "M_CPD__45__8462_c" "M_BUTANAL_c" "M_L__45__CITRULLINE_c" "M_GLYCEROL_c" "M_PRO_c" "M_OLEATE__45__CPD_c" "M_CPD__45__7873_c" "M_SER_c" "M_CHLOROPHYLL__45__A_c" "M_CPD__45__348_c" "M_VAL_c" "M_ACET_c" "M_PHE_c" 29 unproducible targets: "M_LINOLENIC_ACID_c" "M_DOCOSANOATE_c" "M_24__45__METHYLENECHOLESTEROL_c" "M_CPD__45__7834_c" "M_CPD0__45__1264_c" "M_BRASSICASTEROL_c" "M_STIGMASTEROL_c" "M_LINOLEIC_ACID_c" "M_CPD__45__8117_c" "M_CPD__45__843_c" "M_CAMPESTEROL_c" "M_CYCLOARTENOL_c" "M_PENTANOL_c" "M_CPD__45__7872_c" "M_CHOLESTEROL_c" "M_iota__45__Carrageenan_c" "M_LUTEIN_c" "M_nu__45__Carrageenan_c" "M_SITOSTEROL_c" "M_HEPTADECANE__45__CPD_c" "M_CPD__45__12653_c" "M_ICOSAPENTAENOATE_c" "M_CPD__45__9053_c" "M_CPD__45__9288_c" "M_7__45__DEHYDROCHOLESTEROL_c" "M_HYDROXY__45__915__45__DIOXOPROSTA__45__13__45__ENOATE_c" "M_CPD__45__19039_c" "M_CPD__45__8669_c" "M_CPD0__45__1265_c" #Flux Balance Analysis 51/84 compounds with positive flux 33/84 compounds with negative flux GLT_c // GLT_c 1000.0 positive ACETALD_c // ACETALD_c 1000.0 positive GLY_c // GLY_c 1000.0 positive ACET_c // ACET_c 1000.0 positive CPD__45__14553_c // CPD-14553_c 1000.0 positive HIS_c // HIS_c 333.333333333 positive ILE_c // ILE_c 1000.0 positive BUTANOL_c // BUTANOL_c 1000.0 positive LEU_c // LEU_c 1000.0 positive LYS_c // LYS_c 500.0 positive MET_c // MET_c 725.0 positive L__45__ORNITHINE_c // L-ORNITHINE_c 500.0 positive CPD1F__45__129_c // CPD1F-129_c 122.130738523 positive PHE_c // PHE_c 546.610169492 positive ACETONE_c // ACETONE_c 1000.0 positive BENZALDEHYDE_c // BENZALDEHYDE_c 644.554455446 positive ARACHIDONIC_ACID_c // ARACHIDONIC_ACID_c 222.222222222 positive CPD__45__7836_c // CPD-7836_c 333.333333333 positive SER_c // SER_c 1000.0 positive OLEATE__45__CPD_c // OLEATE-CPD_c 250.0 positive THR_c // THR_c 1000.0 positive CPD__45__7845_c // CPD-7845_c 200.0 positive DODECANOATE_c // DODECANOATE_c 400.0 positive BUTANAL_c // BUTANAL_c 1000.0 positive CPD__45__8462_c // CPD-8462_c 285.714285714 positive PALMITATE_c // PALMITATE_c 285.714285714 positive TYR_c // TYR_c 601.941747573 positive CHLOROPHYLL__45__A_c // CHLOROPHYLL-A_c 77.3712621026 positive CPD__45__348_c // CPD-348_c 285.714285714 positive STEARIC_ACID_c // STEARIC_ACID_c 250.0 positive HEXANAL_c // HEXANAL_c 1000.0 positive ARACHIDIC_ACID_c // ARACHIDIC_ACID_c 222.222222222 positive CPD__45__7031_c // CPD-7031_c 1000.0 positive CPD__45__14646_c // CPD-14646_c 122.130738523 positive CPD1F__45__130_c // CPD1F-130_c 119.273879142 positive VAL_c // VAL_c 1000.0 positive CPD__45__9245_c // CPD-9245_c 250.0 positive ETOH_c // ETOH_c 1000.0 positive D__45__Galactosyl__45__12__45__diacyl__45__glycerols_c // D-Galactosyl-12-diacyl-glycerols_c 500.0 positive CPD__45__7875_c // CPD-7875_c 333.333333333 positive ARG_c // ARG_c 250.0 positive METHYLBUT__45__CPD_c // METHYLBUT-CPD_c 1000.0 positive L__45__ASPARTATE_c // L-ASPARTATE_c 1000.0 positive CPD__45__18778_c // CPD-18778_c 500.0 positive L__45__CITRULLINE_c // L-CITRULLINE_c 333.333333333 positive CPD__45__7873_c // CPD-7873_c 250.0 positive PRO_c // PRO_c 1000.0 positive CYSTINE_c // CYSTINE_c 395.66395664 positive GLYCEROL_c // GLYCEROL_c 1000.0 positive ISOBUTYRATE_c // ISOBUTYRATE_c 1000.0 positive TETRACOSANOATE_c // TETRACOSANOATE_c 181.818181818 positive CPD__45__843_c // CPD-843_c 0.0 negative CPD0__45__1265_c // CPD0-1265_c 0.0 negative CYCLOARTENOL_c // CYCLOARTENOL_c 0.0 negative CHOLESTEROL_c // CHOLESTEROL_c 0.0 negative 7__45__DEHYDROCHOLESTEROL_c // 7-DEHYDROCHOLESTEROL_c 0.0 negative BRASSICASTEROL_c // BRASSICASTEROL_c 0.0 negative CAMPESTEROL_c // CAMPESTEROL_c 0.0 negative 24__45__METHYLENECHOLESTEROL_c // 24-METHYLENECHOLESTEROL_c 0.0 negative SITOSTEROL_c // SITOSTEROL_c 0.0 negative PENTANOL_c // PENTANOL_c 0.0 negative STIGMASTEROL_c // STIGMASTEROL_c 0.0 negative HYDROXY__45__915__45__DIOXOPROSTA__45__13__45__ENOATE_c // HYDROXY-915-DIOXOPROSTA-13-ENOATE_c 0.0 negative LUTEIN_c // LUTEIN_c 0.0 negative CPD__45__7830_c // CPD-7830_c 0.0 negative iota__45__Carrageenan_c // iota-Carrageenan_c 0.0 negative L__45__ALPHA__45__ALANINE_c // L-ALPHA-ALANINE_c 0.0 negative nu__45__Carrageenan_c // nu-Carrageenan_c 0.0 negative DOCOSANOATE_c // DOCOSANOATE_c 0.0 negative CPD__45__9288_c // CPD-9288_c 0.0 negative CPD__45__7834_c // CPD-7834_c 0.0 negative MEK_c // MEK_c 0.0 negative CPD__45__8669_c // CPD-8669_c 0.0 negative CPD__45__681_c // CPD-681_c 0.0 negative LINOLEIC_ACID_c // LINOLEIC_ACID_c 0.0 negative HEPTADECANE__45__CPD_c // HEPTADECANE-CPD_c 0.0 negative LINOLENIC_ACID_c // LINOLENIC_ACID_c 0.0 negative CPD__45__19039_c // CPD-19039_c 0.0 negative CPD__45__8117_c // CPD-8117_c 0.0 negative CPD__45__12653_c // CPD-12653_c 0.0 negative CPD__45__7872_c // CPD-7872_c 0.0 negative ICOSAPENTAENOATE_c // ICOSAPENTAENOATE_c 0.0 negative CPD0__45__1264_c // CPD0-1264_c 0.0 negative CPD__45__9053_c // CPD-9053_c 0.0 negative ############# Computing optimization Testing reaction BIOMASS__45__RXN Growth rate: 0.0 Status: optimal IN FLUXES OUT FLUXES OBJECTIVES ----------- ------------ ------------------ BIOMASS__45_... 0 31/33 compounds with positive flux 2/33 compounds with negative flux GLT_c // GLT_c 43.2525951557 positive LEU_c // LEU_c 43.2525951557 positive G3P_c // G3P_c 43.2525951557 positive L__45__ASPARTATE_c // L-ASPARTATE_c 43.2525951557 positive 2__45__KETOGLUTARATE_c // 2-KETOGLUTARATE_c 43.2525951557 positive VAL_c // VAL_c 43.2525951557 positive GAP_c // GAP_c 43.2525951557 positive NADH_c // NADH_c 52.0426749935 positive LYS_c // LYS_c 43.2525951557 positive ATP_c // ATP_c 43.2525951557 positive GLY_c // GLY_c 43.2525951557 positive PHE_c // PHE_c 43.2525951557 positive CYS_c // CYS_c 43.2525951557 positive GLN_c // GLN_c 43.2525951557 positive THR_c // THR_c 43.2525951557 positive HIS_c // HIS_c 43.2525951557 positive PRO_c // PRO_c 43.2525951557 positive PHOSPHO__45__ENOL__45__PYRUVATE_c // PHOSPHO-ENOL-PYRUVATE_c 43.2525951557 positive ERYTHROSE__45__4P_c // ERYTHROSE-4P_c 43.2525951557 positive ACETYL__45__COA_c // ACETYL-COA_c 43.2525951557 positive TYR_c // TYR_c 43.2525951557 positive TRP_c // TRP_c 43.2525951557 positive ILE_c // ILE_c 43.2525951557 positive ASN_c // ASN_c 43.2525951557 positive SER_c // SER_c 43.2525951557 positive SUC__45__COA_c // SUC-COA_c 43.2525951557 positive PYRUVATE_c // PYRUVATE_c 43.2525951552 positive OXALACETIC_ACID_c // OXALACETIC_ACID_c 43.2525951557 positive ARG_c // ARG_c 43.2525951557 positive MET_c // MET_c 43.2525951557 positive RIBOSE__45__5P_c // RIBOSE-5P_c 43.2525951557 positive GLC__45__6__45__P_c // GLC-6-P_c 0.0 negative L__45__ALPHA__45__ALANINE_c // L-ALPHA-ALANINE_c 0.0 negative