Difference between revisions of "Workflow"
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− | == | + | ==Command sequence== |
− | * | + | * '''Check input''': |
− | ** | + | ''Check the validity, consistency and presence of input files'' |
− | *** | + | * '''Annotation based reconstruction''': |
− | + | ''Extract network data from Pathway Tools annotation output.'' | |
− | * | + | * '''Orthology based reconstruction''': |
− | * | + | ''Run the orthology based reconstruction.'' |
− | + | * '''SBML mapping''': | |
− | + | ''Map an SBML file (all the entities IDs) to a reference database (specified in config.txt).'' | |
− | + | * '''Create draft network''': | |
− | + | ''Merges all available networks from the /networks directory into one metabolic network.<BR>Merge all data on the studied species.'' | |
− | + | * '''Manual curation''': | |
− | + | ''Apply the curation described in the form file 1_cycRxns_to_add.csv.'' | |
− | + | * '''Manual curation''': | |
− | + | ''Apply the curation described in the form file 1_keggRxns_to_add.csv.'' | |
+ | * '''Manual curation''': | ||
+ | ''Apply the curation described in the form file 1_reaction_to_delete.csv.'' | ||
+ | * '''Set medium''': | ||
+ | ''Set the growth medium for the studied species.'' | ||
+ | * '''Run gap-filling''': | ||
+ | ''Calculate the gap-filling solution and generate the metabolic network, completed with the gap-filling solution.'' | ||
+ | * '''Get compartments''': | ||
+ | ''Display all the compartments of the metabolic network.'' | ||
+ | * '''Change compartment''': | ||
+ | ''Modify a compartment in the metabolic network.'' | ||
+ | * '''Change compartment''': | ||
+ | ''Modify a compartment in the metabolic network.'' | ||
+ | * '''Change compartment''': | ||
+ | ''Modify a compartment in the metabolic network.'' | ||
+ | * '''Change compartment''': | ||
+ | ''Modify a compartment in the metabolic network.'' | ||
+ | * '''Change compartment''': | ||
+ | ''Modify a compartment in the metabolic network.'' | ||
+ | * '''Change compartment''': | ||
+ | ''Modify a compartment in the metabolic network.'' | ||
+ | * '''Manual curation''': | ||
+ | ''Apply the curation described in the form file 2_cycRxns_to_add.csv.'' | ||
+ | * '''Manual curation''': | ||
+ | ''Apply the curation described in the form file 2_lightRxns_to_add.csv.'' | ||
+ | * '''Manual curation''': | ||
+ | ''Apply the curation described in the form file 2_biomass_rxn.csv.'' | ||
+ | * '''Set FBA''': | ||
+ | ''Set the biomass reaction to run flux balance analysis on the network.'' | ||
+ | * '''Test FBA''': | ||
+ | ''Run flux balance analysis on the network.'' | ||
+ | * '''Create Wiki pages''': | ||
+ | ''Create Wiki pages to display the metabolic network.'' | ||
+ | ==Downloads== | ||
+ | You can download the [[MEDIA:log.txt|command log file here]] |
Latest revision as of 20:02, 21 March 2018
Workflow command history
Command sequence
- Check input:
Check the validity, consistency and presence of input files
- Annotation based reconstruction:
Extract network data from Pathway Tools annotation output.
- Orthology based reconstruction:
Run the orthology based reconstruction.
- SBML mapping:
Map an SBML file (all the entities IDs) to a reference database (specified in config.txt).
- Create draft network:
Merges all available networks from the /networks directory into one metabolic network.
Merge all data on the studied species.
- Manual curation:
Apply the curation described in the form file 1_cycRxns_to_add.csv.
- Manual curation:
Apply the curation described in the form file 1_keggRxns_to_add.csv.
- Manual curation:
Apply the curation described in the form file 1_reaction_to_delete.csv.
- Set medium:
Set the growth medium for the studied species.
- Run gap-filling:
Calculate the gap-filling solution and generate the metabolic network, completed with the gap-filling solution.
- Get compartments:
Display all the compartments of the metabolic network.
- Change compartment:
Modify a compartment in the metabolic network.
- Change compartment:
Modify a compartment in the metabolic network.
- Change compartment:
Modify a compartment in the metabolic network.
- Change compartment:
Modify a compartment in the metabolic network.
- Change compartment:
Modify a compartment in the metabolic network.
- Change compartment:
Modify a compartment in the metabolic network.
- Manual curation:
Apply the curation described in the form file 2_cycRxns_to_add.csv.
- Manual curation:
Apply the curation described in the form file 2_lightRxns_to_add.csv.
- Manual curation:
Apply the curation described in the form file 2_biomass_rxn.csv.
- Set FBA:
Set the biomass reaction to run flux balance analysis on the network.
- Test FBA:
Run flux balance analysis on the network.
- Create Wiki pages:
Create Wiki pages to display the metabolic network.
Downloads
You can download the command log file here